1. Overview:

DON belongs to the single-endomycin family of molds and is produced by certain Fusarium fungi. 3-ADON and 15-ADON are derivatives of DON. These toxins are mostly distributed in grain seeds such as wheat, barley, and corn. Due to their high cytotoxicities and immunosuppressive properties, they pose a health threat to humans and animals 

2. Principle: 

The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the toxins in the sample are extracted, filtered, and passed slowly through the immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. The toxin is then eluted, blown dry with nitrogen, redissolved and then injected into the analytical instrument for detection.

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